Gt(ROSA)26Sor^{em1(CAG-RFP)Maik}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-RFP)Maik}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Masahito Ikawa
• MGI ID: MGI:7431259
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-RFP)Maik} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332932
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A CAG-loxP-Neo-loxP-RFP cassette was inserted via CRISPR/cas9 endonuclease-mediated genome editing in the ES cells. (J:332932)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:332932 Ozawa M, et al., Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice. Sci Rep. 2022 Dec 13;12(1):21558
• All: 1 reference(s)