Gt(ROSA)26Sor^{tm1(CAG-Mlxipl*)Mher}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Mlxipl*)Mher}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Mark A Herman
• MGI ID: MGI:7428861
• Synonyms: 3xFlag-tagged ChREBPbeta, Gt(ROSA)26Sor^{tm1(CAG-Mlxipl)Dks}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Mlxipl*)Mher} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:327368
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, No functional change)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Mlxipl*)Mher} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mlxipl (MGI:1927999)

• Mutation details: The targeting construct inserted into the locus an initial FRT site, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to three repeats of the SV40 polyA signal), 3xFlag-tagged truncated Mxlipl cDNA (coding the ChREBPbeta isoform but not the full-length 3ChREBP alpha isoform), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine growth hormone polyadenylation (bGHpA) signal and a second FRT site. The construct also contains an attB/attP-flanked neomycin cassette. This targeting vector was inserted into intron 1 of the Gt(ROSA)26Sor locus. (J:327368)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:327368 Katz LS, et al., Maladaptive positive feedback production of ChREBPbeta underlies glucotoxic beta-cell failure. Nat Commun. 2022 Jul 30;13(1):4423
• All: 1 reference(s)