Kcnip2^{tm1.1(cre)Uze}
Targeted Allele Detail

Summary

• Symbol: Kcnip2^{tm1.1(cre)Uze}
• Name: Kv channel-interacting protein 2; targeted mutation 1.1, University of Zurich
• MGI ID: MGI:7427724
• Synonyms: Kcnip2^roxCre
• Gene: Kcnip2 Location: Chr19:45780785-45804948 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband D1
• Alliance: Kcnip2^{tm1.1(cre)Uze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332594
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Recombinase)
• Mutation: Insertion
• Kcnip2^{tm1.1(cre)Uze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Kcnip2 (MGI:2135916)

• Mutation details: The targeting construct incorporated the sequence of an optimized roxed-Cre recombinase sequence preceded by the sequence coding for a short self-cleaving peptide P2A, and followed by an FRT-flanked neomycin resistance gene (Neo) into the third coding exon of the Kcnip2 gene. The insert carries an intersectional Dre-dependent Cre recombinase sequence achieved through the inclusion of a STOP cassette flanked by a pair of rox sites into the cre sequence. In presence of dre recombinase, cre recombinase is expressed driven by Kcnip2. The neo-selection sequence was removed by crossing chimeric mice with Flpe deleter mice. (J:332594)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kcnip2 (mouse) Summary of all recombinase alleles driven by Kcnip2. Alliance mouse Kcnip2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnip2 Mutation: 28 strains or lines available

References

• Original: J:332594 Albisetti GW, et al., Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold. Neuron. 2023 Jan 4;111(1):92-105.e5
• All: 1 reference(s)