Ruvbl2^em1(IMPC)Bay
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ruvbl2^em1(IMPC)Bay
• Name: RuvB-like AAA ATPase 2; endonuclease-mediated mutation 1, Baylor College of Medicine
• MGI ID: MGI:7425955
• Synonyms: Ruvbl2^-
• Gene: Ruvbl2 Location: Chr7:45071320-45084817 bp, - strand Genetic Position: Chr7, 29.3 cM, cytoband B2
• Alliance: Ruvbl2^em1(IMPC)Bay page
• IMPC: Ruvbl2 gene page

Ruvbl2^em1(IMPC)Bay/Ruvbl2^em1(IMPC)Bay mice exhibit embryonic lethality, with embryos recovered at E3.5 as blastocysts but not at E7.5. Blastocysts hatch from the zona pellucida but form irregular outgrowths with poorly defined inner cell mass colony when cultured in vitro.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at Baylor College of Medicine using Cas9 and guides with spacer sequences CCGTGTCTATTAGGTCTGCC and TGGCTCATTCGGCCGGGCAT that targeted exon(s) ENSMUSE00000268045.4 ENSMUSE00000268050.4. This resulted in deletion(s) of 731 bp (location: Chr7:45077592-45078322; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ruvbl2 Mutation: 28 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 4 reference(s)