Cebpa^em1Ngwu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cebpa^em1Ngwu
• Name: CCAAT/enhancer binding protein alpha; endonuclease-mediated mutation 1, Ning Wu
• MGI ID: MGI:7424954
• Synonyms: C/EBPalpha HA KI
• Gene: Cebpa Location: Chr7:34818718-34821353 bp, + strand Genetic Position: Chr7, 21.02 cM
• Alliance: Cebpa^em1Ngwu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: crRNA/tracrRNA duplex gRNA is designed to insert an N-terminal HA tag immediately downstream of the start codon. This insertion is designed to only tag the longest isoform, and has slightly less protein expression of the longer isoform than the endogenous protein. (J:332404)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cebpa Mutation: 18 strains or lines available

References

• Original: J:332404 Lau KH, et al., PPARgamma and C/EBPalpha response to acute cold stress in brown adipose tissue. iScience. 2023 Jan 20;26(1):105848
• All: 1 reference(s)