Cnr2^tm1.2Geno
Targeted Allele Detail

Summary

• Symbol: Cnr2^tm1.2Geno
• Name: cannabinoid receptor 2; targeted mutation 1.2, Genoway
• MGI ID: MGI:7424821
• Synonyms: CB2^-
• Gene: Cnr2 Location: Chr4:135622705-135647518 bp, + strand Genetic Position: Chr4, 68.01 cM
• Alliance: Cnr2^tm1.2Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:273502
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An enhanced green fluorescent protein reporter gene (EGFP) preceded by an internal ribosomal entry site (IRES) and followed by an FRT-flanked neomycin gene was inserted into the 3 untranslated region (UTR) of Cnr2. This results in the expression of the EGFP reporter under the control of the endogenous mouse Cnr2 promoter and transcript from the same bicistronic mRNA as the CB2R protein. In addition, exon 3 and the reporter (including the neomycin) are flanked by loxP sites, allowing for conditional inactivation of the gene in cells expressing cre recombinase. The neomycin gene was removed via flp-mediated recombination. Exon 3 and the EGFP were deleted via cre-mediated recombination. (J:273502)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnr2 Mutation: 24 strains or lines available

References

• Original: J:273502 Lopez A, et al., Cannabinoid CB2 receptors in the mouse brain: relevance for Alzheimer's disease. J Neuroinflammation. 2018 May 24;15(1):158
• All: 1 reference(s)