Dnmt3a^em3Mago
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnmt3a^em3Mago
• Name: DNA methyltransferase 3A; endonuclease-mediated mutation 3, Margaret A Goodell
• MGI ID: MGI:7414391
• Synonyms: Dnmt3a2^-
• Gene: Dnmt3a Location: Chr12:3856007-3964443 bp, + strand Genetic Position: Chr12, 1.99 cM, cytoband A2-A3
• Alliance: Dnmt3a^em3Mago page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology deleted the intronic promoter (including the first noncoding exon) of isoform 2 generating a knock-out of isoform 2 (Dnmt3a2). Western blot analysis confirmed the absence of isoform 2 protein in thymus and brain without affecting expression of isoform 1. (J:331942)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3a Mutation: 140 strains or lines available

References

• Original: J:331942 Gu T, et al., The disordered N-terminal domain of DNMT3A recognizes H2AK119ub and is required for postnatal development. Nat Genet. 2022 May;54(5):625-636
• All: 1 reference(s)