Atp2a2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Atp2a2^{tm1c(EUCOMM)Hmgu}
• Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7411257
• Synonyms: Atp2a2^flox
• Gene: Atp2a2 Location: Chr5:122591576-122640288 bp, - strand Genetic Position: Chr5, 62.38 cM, cytoband F
• Alliance: Atp2a2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Atp2a2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:317091
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 122626799 of Chromosome 5 upstream of the critical exon 12 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 12 at position 122627905. The critical exon 12 is thus flanked by loxP sites. The lacZ and neomycin resistance gene were removed via flp-mediated recombination generating a conditional ready (floxed) allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:317091)

Expression

In Mice Carrying this Mutation:

8 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp2a2 Mutation: 74 strains or lines available

References

• Original: J:317091 Nakajima K, et al., Brain-specific heterozygous loss-of-function of ATP2A2, endoplasmic reticulum Ca2+ pump responsible for Darier's disease, causes behavioral abnormalities and a hyper-dopaminergic state. Hum Mol Genet. 2021 Aug 28;30(18):1762-1772
• All: 2 reference(s)