Tg(tetO-GCaMP8s)1Genie
Transgene Detail

Summary

• Symbol: Tg(tetO-GCaMP8s)1Genie
• Name: transgene insertion 1, Janelia GENIE Project Team
• MGI ID: MGI:7410925
• Synonyms: Tet-jCAMP8s-01
• Transgene: Tg(tetO-GCaMP8s)1Genie Location: unknown
• Alliance: Tg(tetO-GCaMP8s)1Genie page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inducible, Reporter)
• Mutation: Insertion
• Mutation details: The transgenic construct contains a tetO (pTRE-Tight) promoter driving expression of a green fluorescent calcium indicator, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), and a polyadenylation sequence (pA). The GCaMP8s (sensitive) incorporates a hexa-histidine affinity tag, an endothelial nitric oxide synthase peptide (ENOSP; modified with N19T, S24I, and S26M), a circularly permutated green fluorescent protein (cpGFP; modified with Q88E), the rat calcium-binding protein calmodulin (CaM; modified with F286Y and Q315H), and LKI amino acid linkers that replace the RS20 (previously referred to as M13) linkers. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 3 reference(s)