Gt(ROSA)26Sor^{tm4(CAG-cas9*/GAG-POL,-mNeonGreen)Tyj}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(CAG-cas9*/GAG-POL,-mNeonGreen)Tyj}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Tyler Jacks
• MGI ID: MGI:7408317
• Synonyms: Rosa26^PE2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(CAG-cas9*/GAG-POL,-mNeonGreen)Tyj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:335562
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: B6.129P2-Trp53^tm1Brn

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct contains from 5' to 3' a CAGG promoter, FRT site, loxP-stop-loxP, a human optimized Cas9 with the H840A mutation fused to a MMLV-RT (Moloney murine leukemia virus reverse transcriptase, GAG-POL), P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA. The cre-inducible cas9*/ GAG-POL fusion construct is referred to as the primer editor enzyme (PE2). The PE2 enzyme complexes with prime editing guide RNAs that can encode a mutation of interest within a reverse transcriptase template. (J:335562)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:335562 Ely ZA, et al., A prime editor mouse to model a broad spectrum of somatic mutations in vivo. Nat Biotechnol. 2023 May 11
• All: 1 reference(s)