Gt(ROSA)26Sor^{tm4(CAG-Hoxa2,-EGFP)Fmr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(CAG-Hoxa2,-EGFP)Fmr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Filippo M Rijli
• MGI ID: MGI:7408304
• Synonyms: ROSA26^{CAG::(lox-stop-lox)-Hoxa2-IRES-EGFP-WPRE)}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(CAG-Hoxa2,-EGFP)Fmr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:320009
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(CAG-Hoxa2,-EGFP)Fmr} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hoxa2 (MGI:96174)

• Mutation details: The following elements were inserted into the ROSA locus: the CAG promoter, a loxP site flanked STOP cassette, a Hoxa2 cDNA tagged with a 3xFLAG, an IRES-EGFP, a WPRE element, a bGH poly(A) and a PGK-neomycin cassette. Only after cre-mediated removal of the STOP cassette will this allele express the Hoxa2 and EGFP genes. (J:320009)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1205 strains or lines available

References

• Original: J:320009 Kitazawa T, et al., Different Ectopic Hoxa2 Expression Levels in Mouse Cranial Neural Crest Cells Result in Distinct Craniofacial Anomalies and Homeotic Phenotypes. J Dev Biol. 2022;10
• All: 1 reference(s)