Rangrf^tm1.1Tke
Targeted Allele Detail

Summary

• Symbol: Rangrf^tm1.1Tke
• Name: RAN guanine nucleotide release factor; targeted mutation 1.1, Tie Ke
• MGI ID: MGI:7386934
• Synonyms: Mog1^-
• Gene: Rangrf Location: Chr11:68863310-68866011 bp, - strand Genetic Position: Chr11, 42.24 cM, cytoband B3
• Alliance: Rangrf^tm1.1Tke page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:325055
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: Three premature stop codons, V31* (GTG to TAG), P32* (CCA to TGA), and D33* (GAC to TAA) were introduced in exon 2, which is expected to result in nonsense-mediated decay of mRNA, along with a loxP-flanked neomycin resistance gene following the mutated exon 2. The selection cassette was removed via cre-mediated recombination. This knock-in strategy was used to knock-out the gene without affecting the partially overlapping Slc25a35 gene (transcribed in opposite direction). RT-qPCR analysis shows 90% reduction in cardiac mRNA of homozygotes. Western blot analysis shows nearly complete loss of expression in homozygous heart. (J:325055)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rangrf Mutation: 10 strains or lines available

References

• Original: J:325055 Zhao M, et al., Mog1 deficiency promotes cardiac contractile dysfunction and isoproterenol-induced arrhythmias associated with cardiac fibrosis and Cx43 remodeling. Biochim Biophys Acta Mol Basis Dis. 2022 May 6;1868(9):166429
• All: 1 reference(s)