Gt(ROSA)26Sor^{tm8.3(CAG-tdiRFP*)Imayo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm8.3(CAG-tdiRFP*)Imayo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8.3, Itaru Imayoshi
• MGI ID: MGI:7385051
• Synonyms: Gt(ROSA)26Sor^{tm1.3(CAG-tdiRFP713NLS)Imayo}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm8.3(CAG-tdiRFP*)Imayo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104881
• Parent Cell Line: EGR-101 (ES Cell)
• Strain of Origin: C57BL/6NSlc

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced the tdiRFP713NLS reporter gene expressed under CAG promoter and WPRE sequence into 3 UTR region. Both cre- and flp-mediated recombination is required to achieve expression of the reporter. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain. Then, loxP-Stop-loxP cassette was removed by injecting pIC-Cre expression plasmid to the fertilized eggs. Therefore, tdiFP713NLS is expressed by all cells throughout the body. The HA-epitope tga was is attched to tdiFP713NLS. (J:104881)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 1 reference(s)