Gen1^tm1.1Jaych
Targeted Allele Detail

Summary

• Symbol: Gen1^tm1.1Jaych
• Name: GEN1, Holliday junction 5' flap endonuclease; targeted mutation 1.1, Jayanta Chaudhuri
• MGI ID: MGI:7378512
• Synonyms: Gen1^-, Gen1^tm1M
• Gene: Gen1 Location: Chr12:11288921-11315802 bp, - strand Genetic Position: Chr12, 5.58 cM
• Alliance: Gen1^tm1.1Jaych page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:330401
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Not Specified
• Mutation details: The allele was generated by replacing exon 4 of the Gen1 gene on chromosome 12 with a loxP-flanked neomycin cassette. The construct was electroporated into embryonic stem (ES) cells. The floxed neomycin cassette was removed via transient expression of Cre recombinase. (J:101977, J:330401)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gen1 Mutation: 54 strains or lines available

References

• Original: J:330401 Fernandez KC, et al., The structure-selective endonucleases GEN1 and MUS81 mediate complementary functions in safeguarding the genome of proliferating B lymphocytes. Elife. 2022 Oct 3;11:e77073
• All: 2 reference(s)