Fitm1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fitm1^em1(IMPC)J
• Name: fat storage-inducing transmembrane protein 1; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:7367236
• Gene: Fitm1 Location: Chr14:55813131-55814409 bp, + strand Genetic Position: Chr14, 28.19 cM
• Alliance: Fitm1^em1(IMPC)J page
• IMPC: Fitm1 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTGAGGACTGACGGTCAACA and GTACGTGACTTCCATCCTGT, which resulted in a 1578 bp deletion beginning at Chromosome 14 position 55,575,498 bp and ending after 55,577,075 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000124452 and ENSMUSE00000317360 (exons 1 and 2) and 607 bp of flanking intronic sequence including the start site, splice acceptor and donor and is predicted to result in a null allele. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fitm1 Mutation: 14 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 1 reference(s)