Gt(ROSA)26Sor^{tm13(Nfkb-tdTomato)Nat}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm13(Nfkb-tdTomato)Nat}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 13, Jeremy Nathans
• MGI ID: MGI:7365228
• Synonyms: NFKB-LSL-nls-tdT-3xHA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm13(Nfkb-tdTomato)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: Five tandem NF-kappa-B (Nfkb) response elements (GGGAATTTCC) are inserted upstream of a chicken minimal delta crystallin promoter (Crygd) followed by an intron. This is followed by a loxP-transcription stop-loxP (LSL) cassette, a nuclear localized tdTomato (tdT) with a triple hemagglutinin (HA) epitope tag, and a bovine growth hormone (bGH) in the 3' untranslated region. An FRT-Neomycin-FRT selection cassette is retained immediately 5'; of the five tandem NF-kappa-B binding sites. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)