Gt(ROSA)26Sor^{tm13.1(CAG-HRP)Luo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm13.1(CAG-HRP)Luo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 13.1, Liqun Luo
• MGI ID: MGI:7346148
• Synonyms: Cre-iPEEL
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm13.1(CAG-HRP)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:329532
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: A cassette incorporating CAG-FRT-stop-FRT(FSF)-loxP-stop-loxP(LSL)-SP-hemagglutinin (HA)-HRPtm-WPRE-bGHpolyA flanked by attB sites is introduced to the first intron of Gt(ROSA)26Sor by integrase-mediated transgenesis. The CAG promoter, FSF, and LSL cassettes (incorporating poly(A) transcriptional terminal signals and translational stop codons in all three reading frames from the Ai65 targeting vector) enable high levels of HRP expression gated by Flp and Cre recombinases. The HRP coding DNA sequence was preceded by an IgK signal peptide (SP) and a hemagglutinin (HA) tag and followed by a PDGFRbeta transmembrane (tm) domain and a short intracellular domain with Kir2.1 (Kcnj2) trafficking and endoplasmic reticulum (ER) transport signals separated by Gly-Ser-Gly (GSG) linkers. The whole coding sequence was codon optimized and followed by a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and a bovine growth hormone polyadenylation signal (bGHpolyA). Flp-mediated recombination removed the FRT-flanked stop cassette. (J:329532)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Gt(ROSA)26Sor^tm12Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺)-derived R1 ES cells.

References

• Original: J:329532 Shuster SA, et al., In situ cell-type-specific cell-surface proteomic profiling in mice. Neuron. 2022 Oct 6
• All: 1 reference(s)