Ctnna3^em1Cgot
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ctnna3^em1Cgot
• Name: catenin alpha 3; endonuclease-mediated mutation 1, Cara Gottardi
• MGI ID: MGI:7343802
• Synonyms: Ctnna3^eGFPcaax, Ctnna3^GFP, Ctnna3^KO
• Gene: Ctnna3 Location: Chr10:63265877-64839446 bp, + strand Genetic Position: Chr10, 32.57 cM
• Alliance: Ctnna3^em1Cgot page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:332176
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing is used to insert a 5' HA tag, a membrane anchored eGFP repair plasmid (eGFPcaax), a STOP sequence, a bGH polyA sequence and a 3' HA tag into the 3' end of the exon2 locus, and a non-targeting PGK-puromycin plasmid. The endogenous promoter drives eGFP expression. (J:332176)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ctnna3 Mutation: 45 strains or lines available

References

• Original: J:332176 Weng A, et al., Alpha-T-catenin is expressed in peripheral nerves as a constituent of Schwann cell adherens junctions. Biol Open. 2022 Dec 15;11(12):bio059634
• All: 4 reference(s)