Cobl^tm1.1Bqu
Targeted Allele Detail

Summary

• Symbol: Cobl^tm1.1Bqu
• Name: cordon-bleu WH2 repeat; targeted mutation 1.1, Britta Qualmann
• MGI ID: MGI:7343594
• Gene: Cobl Location: Chr11:12186676-12415022 bp, - strand Genetic Position: Chr11, 7.3 cM
• Alliance: Cobl^tm1.1Bqu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271615
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site and a PKG-neomycin resistance cassette flanked by FRT sites were inserted in intron 10 and a second loxP site was inserted in intron 11. Cre-mediated recombination removed exon 11 and the selection cassette. Exon 11 was targeted for deletion to eradicate specifically all actin nucleating products of the gene but not the putative truncated form which lacks all actin-binding WH2 domains and would have functions unrelated to actin nucleation. However, no truncated protein is detected in the brain, indicating a knock-out allele. (J:271615)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cobl Mutation: 78 strains or lines available

References

• Original: J:271615 Haag N, et al., The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea. Cell Rep. 2018 Aug 28;24(9):2418-2431.e6
• All: 3 reference(s)