Rr270^em1Mgn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr270^em1Mgn
• Name: regulatory region 270; endonuclease-mediated mutation 1, Mark A Magnuson
• MGI ID: MGI:7339168
• Synonyms: deltaCR1, Sox17^em1Mgn
• Gene: Rr270 Location: unknown Genetic Position: Chr1, Syntenic
• Alliance: Rr270^em1Mgn page

Mutation origin

• Strain of Origin: CD-1

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: The conserved region, upstream of transcription start site 1 (TSS1) in the 5' UTR of Sox17 exon 1, was targeted with crRNAs (targeting TTTCTTCACTAAATGGGTGTTGG and ACTAGAGATTTACTGGGGTGGGG), tracrRNA and an ssODN template (GATACTTTGGGAGGAGTGTTGGGGCCAACCGCACTTCAGACCCGCCAGCAGTGTGAGTGGGCCATATTTCAGTTTCTTCACTAAATGGTGGGGTGGGGGTGGGTATCTGGACTAGAAGCCTGGCACGCCTGTCTGCTGTAGTCCGCAGCGGTATCACACTCAAACAGTGCAGGATTTCTA) using CRISPR/Cas9 technology, leading to a 117 bp deletion. (J:328045)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rr270 Mutation: 0 strains or lines available

References

• Original: J:328045 Trinh LT, et al., Differential regulation of alternate promoter regions in Sox17 during endodermal and vascular endothelial development. iScience. 2022 Sep 16;25(9):104905
• All: 1 reference(s)