Cyp2u1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyp2u1^{tm1c(EUCOMM)Wtsi}
• Name: cytochrome P450, family 2, subfamily u, polypeptide 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7338926
• Synonyms: Cyp2u1 floxed
• Gene: Cyp2u1 Location: Chr3:131084140-131097806 bp, - strand Genetic Position: Chr3, 60.89 cM, cytoband H1
• Alliance: Cyp2u1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cyp2u1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:328659
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 131091250 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 131092532. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:328659)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp2u1 Mutation: 30 strains or lines available

References

• Original: J:328659 Pujol C, et al., Implication of folate deficiency in CYP2U1 loss of function. J Exp Med. 2021 Nov 1;218(11):e20210846
• All: 1 reference(s)