Islr^{tm2.1(cre/ERT2)Mat}
Targeted Allele Detail

Summary

• Symbol: Islr^{tm2.1(cre/ERT2)Mat}
• Name: immunoglobulin superfamily containing leucine-rich repeat; targeted mutation 2.1, Masahide Takahashi
• MGI ID: MGI:7335171
• Synonyms: Meflin-Cre, Meflin-CreERT2
• Gene: Islr Location: Chr9:58063547-58066563 bp, - strand Genetic Position: Chr9, 31.63 cM
• Alliance: Islr^{tm2.1(cre/ERT2)Mat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280029
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Islr^{tm2.1(cre/ERT2)Mat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Islr (MGI:1349645)

• Mutation details: The CreERT2 cassette replaced the exon 2 of the Islr (Meflin) gene which encode the entire ORF. The Flippase Recognition Target (FRT)-flanked PGK-neo resistance gene was also inserted. Chimeras were mated with Rosa26-FLP mice to remove the neo-cassette. (J:280029)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Islr (mouse) Summary of all recombinase alleles driven by Islr. Comparative matrix view of recombinase activities Alliance mouse Islr gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Islr Mutation: 23 strains or lines available

References

• Original: J:280029 Mizutani Y, et al., Meflin-Positive Cancer-Associated Fibroblasts Inhibit Pancreatic Carcinogenesis. Cancer Res. 2019 Oct 15;79(20):5367-5381
• All: 5 reference(s)