Gt(ROSA)26Sor^{tm1.1(CAG-HL9-MG*V70A,-mBFP,-SNCA,-mTFP1,-SNCA*A30P,-mKO2,-SNCA*A53T)Rali}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-HL9-MG*V70A,-mBFP,-SNCA,-mTFP1,-SNCA*A30P,-mKO2,-SNCA*A53T)Rali}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Rodger A Liddle
• MGI ID: MGI:7332841
• Synonyms: SNCAbow
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-HL9-MG*V70A,-mBFP,-SNCA,-mTFP1,-SNCA*A30P,-mKO2,-SNCA*A53T)Rali} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(CAG-HL9-MG*V70A,-mBFP,-SNCA,-mTFP1,-SNCA*A30P,-mKO2,-SNCA*A53T)Rali} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SNCA (6622)

  Snca	(MGI:1277151)

  contains three SCNA sequences: wild-type, A30P, and A53T

• Mutation details: A targeting vector contains (from 5' to 3') a strong CAG (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid) promoter, three pairs of orthogonal lox sites (LoxN, Lox2272, and LoxP), and the alphasynuclein-Crainbow (SNCAbow) construct (described in greater detail below), followed by an FRT-flanked neomycin resistance (neo) cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The alphasynuclein-Crainbow construct contains three pairs of orthogonal lox sites (LoxN, Lox2272, and LoxP) that separate four cassettes, three of which are associated with a corresponding alpha-synuclein gene sequence. The first cassette expresses a chemically inducible nearinfrared fluorogenactivating peptide (referred to as FAPMars1). Mars1 is a V70A mutation of the human single-chain antibody (scFv)-based fluorogen isolated from HL9-MG (human library 9 malachite green). The next three cassettes encode a unique nuclear localized fluorescent protein (TagBFP:blue, mTFP1:turquoise, or mKO2:orange) and a corresponding human alpha-synuclein protein sequence (SNCA(WT), SNCA(A30P), or SNCA(A53T), respectively) each separated by a P2A peptide to mediate ribosomal skipping. Each alpha-synuclein sequence contains a C-terminal epitope tag: SNCA(WT) tagged with V5, SNCA(A30P) tagged with 3XHA, or SNCA(A53T) tagged with Myc. To ensure that only one fluorescent protein is expressed at any one time, an SV40 polyadenylation signal was inserted downstream of each open reading frame. Upon cre activation, the lox sites recombine to create three mutually exclusive excision possibilities with the fluorescent protein that is placed first after the CAG promoter being expressed. FLP-mediated recombination removed the neomycin cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 956 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)