Igs7tm224(CAG-EGFP,CAG-dTomato)Tasic
Targeted Allele Detail
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Symbol: |
Igs7tm224(CAG-EGFP,CAG-dTomato)Tasic |
Name: |
intergenic site 7; targeted mutation 224, Bosiljka Tasic |
MGI ID: |
MGI:7331594 |
Synonyms: |
Ai224, Ai224 (TICL-nls-EGFP-ICF-nls-dTom), Igs7tm224(CAG-EGFP,CAG-dTomato)Hze |
Gene: |
Igs7 Location: unknown Genetic Position: Chr9, Syntenic
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Alliance: |
Igs7tm224(CAG-EGFP,CAG-dTomato)Tasic page
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Allele Type: |
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Targeted (Conditional ready, Reporter) |
Mutation: |
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Insertion
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Mutation details: The targeting vector contains from (from 5' to 3') a Bxb1 attB site, a partial green fluorescent protein sequence (non-functional 500bp fragment to increase the space between the Bxb1 attB site and the insulator sequence to improve stability of the targeting vector), two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), a synthetic enhanced green fluorescent protein sequence (EGFP) with a nuclear localization sequence, a BGH polyA, two copies of chicken beta-globin HS4 insulator element, the CAG promoter, an FRT-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), a dTomato sequence with a nuclear localization sequence (tdT; encoding the non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein [tandem dimer]), a BGH polyA, a PhiC31 attB site, a PGK-5'hygro cassette, an RNA splice donor, a Bxb1 attR site, SA, 3'hygro, SV40pA, and PhiC31 attP.
(J:101977)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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