Ucp1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ucp1^{tm1c(EUCOMM)Hmgu}
• Name: uncoupling protein 1 (mitochondrial, proton carrier); targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7328587
• Synonyms: Ucp1^tm1c
• Gene: Ucp1 Location: Chr8:84016981-84025081 bp, + strand Genetic Position: Chr8, 39.65 cM
• Alliance: Ucp1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ucp1 gene page

Mutation origin

• Mutant Cell Line: HEPD0676_4_E07
• Germline Transmission: Earliest citation of germline transmission: J:322822
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 84017660 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 84018453. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:322822)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ucp1 Mutation: 51 strains or lines available

References

• Original: J:322822 Dieckmann S, et al., Susceptibility to diet-induced obesity at thermoneutral conditions is independent of UCP1. Am J Physiol Endocrinol Metab. 2022 Feb 1;322(2):E85-E100
• All: 4 reference(s)