Tg(JAK2*)N1Rsko
Transgene Detail

Summary

• Symbol: Tg(JAK2*)N1Rsko
• Name: transgene insertion N1, Radek C Skoda
• MGI ID: MGI:7327623
• Synonyms: JAK2-N542-E543del
• Transgene: Tg(JAK2*)N1Rsko Location: unknown
• Alliance: Tg(JAK2*)N1Rsko page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(JAK2*)N1Rsko expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  JAK2 (3717)

  Jak2	(MGI:96629)

  human JAK2 gene modified to include the deletion of N542 and E543 (AATGAA)

• Mutation details: The transgenic construct contains exons 1-12 from a BAC carrying the human JAK2 gene modified to include the deletion of N542 and E543 (AATGAA) cloned from a polycythemia vera (PV) patient, an antiparallel lox66 site and a lox71 site flanking exon 12, a partial cDNA encoding JAK2 exons 13-25, and a polyA signal. The segment between the lox sites was inverted by restriction enzyme digest, creating an inducible Flip-Flop transgene. In the flipped configuration, the full length JAK2 protein cannot be made. (J:236259)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:236259 Grisouard J, et al., JAK2 exon 12 mutant mice display isolated erythrocytosis and changes in iron metabolism favoring increased erythropoiesis. Blood. 2016 Aug 11;128(6):839-51
• All: 3 reference(s)