Tg(Mapt-ECFP*/Venus*)#Ito
Transgene Detail

Summary

• Symbol: Tg(Mapt-ECFP*/Venus*)#Ito
• Name: transgene insertion, Shigeyoshi Itohara
• MGI ID: MGI:7327520
• Synonyms: LSL-YC2.60, Tg(Mapt-neo,-YC2.60)#Ito
• Transgene: Tg(Mapt-ECFP*/Venus*)#Ito Location: unknown
• Alliance: Tg(Mapt-ECFP*/Venus*)#Ito page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Tg(Mapt-ECFP*/Venus*)#Ito expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mapt (MGI:97180)

• Mutation details: The transgene contains the mouse promoter driving neuron-specific expression, a floxed stop cassette, the YCX2.60 coding sequence [an enhanced cyan fluorescent protein with C-terminal 11 amino acids deleted (ECFPDeltaC11 with R27K and K213N mutations, a rat calmodulin DNA fragment (CaM; aa 5-148 with N60D and D78Y mutations [to increase the fluorescence change for small calcium transients] and E127V mutation), a GG linker, a chicken smooth muscle M13 fragment of myosin light chain kinase (with I-to-S mutation in the 20th aa of the sequence), and a circularly permutated Venus enhanced yellow fluorescent protein (cp173 Venus; aa 174-239, a 15 bp linker and aa 1-173)], Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 intron, and polyadenylation sequence. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:270642)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:270642 Kuroki S, et al., Excitatory Neuronal Hubs Configure Multisensory Integration of Slow Waves in Association Cortex. Cell Rep. 2018 Mar 13;22(11):2873-2885
• All: 2 reference(s)