Tcfl5^tm1.1Frem
Targeted Allele Detail

Summary

• Symbol: Tcfl5^tm1.1Frem
• Name: transcription factor-like 5 (basic helix-loop-helix); targeted mutation 1.1, Manuel Fresno
• MGI ID: MGI:7316455
• Synonyms: TCFL5^-
• Gene: Tcfl5 Location: Chr2:180263749-180284501 bp, - strand Genetic Position: Chr2, 103.16 cM
• Alliance: Tcfl5^tm1.1Frem page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326601
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The loxP flanked exon 4 was removed via cre-mediated recombination in the germline. Homozygous mutant testes lack the full-length transcript but show the presence of aberrant transcripts. Western blot analysis demonstrated the absence of protein expression in testes. Immunofluorescence analysis confirmed the absence of protein in spermatocytes. (J:326601)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcfl5 Mutation: 12 strains or lines available

References

• Original: J:326601 Galan-Martinez J, et al., TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse. Sci Rep. 2022 Jun 29;12(1):10956
• All: 1 reference(s)