Cry1^em1Liga
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cry1^em1Liga
• Name: cryptochrome circadian regulator 1; endonuclease-mediated mutation 1, Gang Li
• MGI ID: MGI:7314236
• Synonyms: Cry1 KO
• Gene: Cry1 Location: Chr10:84967564-85020918 bp, - strand Genetic Position: Chr10, 41.63 cM, cytoband C
• Alliance: Cry1^em1Liga page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9-based gene editing generated a 22-bp deletion in exon 7, as confirmed by DNA sequencing. Western blot analysis demonstrated the absence of protein expression in testicular lysates from homozygous mutant mice. (J:323111)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cry1 Mutation: 35 strains or lines available

References

• Original: J:323111 Li C, et al., Cry1 deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and immune response in mouse testis. Mol Reprod Dev. 2018 Apr;85(4):325-335
• All: 1 reference(s)