Crbn^tm2.1Ble
Targeted Allele Detail

Summary

• Symbol: Crbn^tm2.1Ble
• Name: cereblon; targeted mutation 2.1, Benjamin L Ebert
• MGI ID: MGI:7312145
• Synonyms: Crbn^V380E/I391V
• Gene: Crbn Location: Chr6:106757162-106777038 bp, - strand Genetic Position: Chr6, 49.24 cM, cytoband E2
• Alliance: Crbn^tm2.1Ble page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326489
• Parent Cell Line: iTL BF1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: V380E (GTG to GAG; exon 10) and I391V (ATT to GTG; exon 11) mutations were introduced to the gene via homologous recombination. A loxP/FRT-flanked neomycin cassette placed in intron 9 was excised in the ES cells expressing FLP recombinase, leaving one set of loxP/FRT sites. The humanized mutations create sensitivity to thalidomide analogues in the mouse. (J:326489)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crbn Mutation: 33 strains or lines available

References

• Original: J:326489 Sellar RS, et al., Degradation of GSPT1 causes TP53-independent cell death in leukemia whilst sparing normal hematopoietic stem cells. J Clin Invest. 2022 Jun 28;:e153514
• All: 1 reference(s)