Rnaseh2a^tm2.1Crh
Targeted Allele Detail

Summary

• Symbol: Rnaseh2a^tm2.1Crh
• Name: ribonuclease H2, large subunit; targeted mutation 2.1, Robert J Crouch
• MGI ID: MGI:7311689
• Synonyms: Rnaseh2a^P40D-Y211A, Rnaseh2a^RED
• Gene: Rnaseh2a Location: Chr8:85683239-85694498 bp, - strand Genetic Position: Chr8, 41.38 cM, cytoband C3
• Alliance: Rnaseh2a^tm2.1Crh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:270825
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting construct introduced mutations that resulted in the amino acid substitutions of proline with aspartic acid at position 40 (P40D) and tyrosine with alanine at position 211 (Y211A). Flp-mediated recombination removed the FRT-flanked hygromycin resistance cassette. (J:270825)

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnaseh2a Mutation: 45 strains or lines available

References

• Original: J:270825 Uehara R, et al., Two RNase H2 Mutants with Differential rNMP Processing Activity Reveal a Threshold of Ribonucleotide Tolerance for Embryonic Development. Cell Rep. 2018 Oct 30;25(5):1135-1145.e5
• All: 1 reference(s)