Epha2^em2Alsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Epha2^em2Alsh
• Name: Eph receptor A2; endonuclease-mediated mutation 2, Alan Shiels
• MGI ID: MGI:7310423
• Synonyms: Epha2^indel722
• Gene: Epha2 Location: Chr4:141028551-141056695 bp, + strand Genetic Position: Chr4, 73.67 cM, cytoband D-E
• Alliance: Epha2^em2Alsh page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (No functional change)
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: CRISPR-targeting of exon 13 produced the off-target insertion of a single base causing a frameshift and the introduction of 3 novel codons, including the targeted 2 bp change (AGG to CAG in codon 722), followed by deletion of 58 bp coding region before returning to the correct reading frame. (J:312486)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epha2 Mutation: 98 strains or lines available

References

• Original: J:312486 Zhou Y, et al., Mutation of the EPHA2 Tyrosine-Kinase Domain Dysregulates Cell Pattern Formation and Cytoskeletal Gene Expression in the Lens. Cells. 2021 Sep 30;10(10)
• All: 1 reference(s)