F9^em1Smoc
Endonuclease-mediated Allele Detail

Summary

• Symbol: F9^em1Smoc
• Name: coagulation factor IX; endonuclease-mediated mutation 1, Shanghai Model Organisms Center
• MGI ID: MGI:7289575
• Gene: F9 Location: ChrX:59044824-59076119 bp, + strand Genetic Position: ChrX, 33.5 cM, cytoband A6-A7
• Alliance: F9^em1Smoc page

Mutation origin

• Strain of Origin: C57BL/6JSmoc

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The F9 gene is located on the X chromosome. In this F9 knockout mouse model, gRNAs were designed targeting Exon8 of F9 gene via CRISPR gene editing technology. Loss of F9 caused coagulopathy in mice. Mice that are homozygous for F9 knockout are viable, fertile and normal in size. Normal fighting in the cage may cause bleeding or even death due to massive internal hemorrhaging. After the tail cutting, wounds must be cauterized to prevent homozygous knockout mice from blood loss and death. This strain is a powerful model for studying coagulopathy, gene therapy methods and function of factor IX mutations. F9-KO mice (Stock No.NM-KO-18046) carry a knockout allele derived from the targeted deletion of exon 8. While F9-KO(2) mice (Stock No.NM-KO-200607) carrying the exon 1-8 deletion. (J:238766)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any F9 Mutation: 9 strains or lines available

References

• Original: J:238766 Shanghai Model Organisms Center, Information obtained from the Shanghai Model Organisms Center (SMOC), Shanghai, China. MGI Direct Data Submission. 2017
• All: 1 reference(s)