Npsr1^em2Yfu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Npsr1^em2Yfu
• Name: neuropeptide S receptor 1; endonuclease-mediated mutation 2, Ying-Hui Fu
• MGI ID: MGI:7284434
• Synonyms: Npsr1^-
• Gene: Npsr1 Location: Chr9:24009292-24227694 bp, + strand Genetic Position: Chr9, 9.99 cM
• Alliance: Npsr1^em2Yfu page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Using an sgRNA (targeting ) and an ssODN template (GTCCAGCCCGTGGCCTCTCACCTGATAATTGCCAAGGGAATGAAGTACACCAGAAAGGCGACGATGGTCATGTACGGGGTCCAGTGCGAGTCATCCGGCCACAGTGCCCAGCACTGCACCTCACCATTGGAAAGTGTCCTTTTCCCAAATATGATCAGCGTGGGAATGGAGA) with CRISPR/Cas9 technology, exon 6 (coding for K161-S227) was deleted as a by-product of the intended p.Y206H mutation. (J:325012)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Npsr1 Mutation: 37 strains or lines available

References

• Original: J:325012 Xing L, et al., Mutant neuropeptide S receptor reduces sleep duration with preserved memory consolidation. Sci Transl Med. 2019 Oct 16;11(514):eaax2014
• All: 1 reference(s)