Myd88^em1.1Rsky
Endonuclease-mediated Allele Detail

Summary

• Symbol: Myd88^em1.1Rsky
• Name: myeloid differentiation primary response gene 88; endonuclease-mediated mutation 1.1, Klaus Rajewsky
• MGI ID: MGI:7281146
• Synonyms: Myd88^L252P, Myd88^LP
• Gene: Myd88 Location: Chr9:119165000-119169084 bp, - strand Genetic Position: Chr9, 71.33 cM
• Alliance: Myd88^em1.1Rsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:308792
• Parent Cell Line: ART B6-3, B6-3 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutations: Insertion, Single point mutation
• Mutation details: Using BAC clones from the RPCIB-731 library and sgRNAs with CRISPR/Cas9 technology, a loxP site plus an extra copy of exon 5 (with a T-to-C mutation changing leucine codon 252 (CTG) to proline (CCG) (p.L252P), and an IRES and the GFP reporter gene after the stop codon), intron 5 and exon 6 was inserted downstream of the gene and a second loxP site was inserted into intron 4. The mutation is the equivalent of the p.L265P mutation, in the TIR domain, found in Waldenstroms macroglobulinemia (WM) and IgM monoclonal gammopathy of undetermined/uncertain significance (IgM MGUS) patients. The mutated exon and the GFP gene will only be expressed after cre-mediated deletion of the floxed endogenous exons 5 and 6. The FRT site flanked neomycin resistance gene cassette that was inserted between the endogenous gene and duplicate exon 5, was removed through subsequent flp-mediated recombination. (J:308792)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myd88 Mutation: 50 strains or lines available

References

• Original: J:308792 Schmidt K, et al., B-Cell-Specific Myd88 L252P Expression Causes a Premalignant Gammopathy Resembling IgM MGUS. Front Immunol. 2020;11:602868
• All: 1 reference(s)