Gt(ROSA)26Sor^{tm1(Uprt)Bara}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Uprt)Bara}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Jonathan Barasch
• MGI ID: MGI:7279147
• Synonyms: Rosa^Uprt
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Uprt)Bara} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:324326
• Parent Cell Line: KV1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Uprt)Bara} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Uprt (MGI:2685620)

• Mutation details: The cDNA of Uprt from Toxoplasma gondii (T. gondii) was inserted into a Rosa26 targeting vector. A knockin vector was created with Uprt cDNA flanked by a floxed-stop cassette. Uprt-knockin mice were generated by standard homologous recombination in mouse embryonic stem (ES) cells. The presence of transcriptional stop cassette PGK-neo-pA-3xpA prevented downstream Uprt cDNA expression, while mating with mice expressing tissue/cell-specific Cre activated the expression of Uprt cDNA in a cell-specific manner. (J:324326)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:324326 Shen TH, et al., Snapshots of nascent RNA reveal cell- and stimulus-specific responses to acute kidney injury. JCI Insight. 2022 Mar 22;7(6):e146374
• All: 2 reference(s)