Tesmin^em2Qsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tesmin^em2Qsh
• Name: testis expressed metallothionein like; endonuclease-mediated mutation 2, Qinghua Shi
• MGI ID: MGI:7276187
• Synonyms: Mtl5^c-mu
• Gene: Tesmin Location: Chr19:3438857-3457823 bp, + strand Genetic Position: Chr19, 3.15 cM, cytoband B
• Alliance: Tesmin^em2Qsh page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 76 bp deletion in exon 9 resulting in a C-terminal truncated protein (p.Ser445Argfs*3). This truncation mutation eliminates TESMINs interaction with LIN9 and consequently leads to cytoplasmic retention of TESMIN in pachytene spermatocytes. Western blot analysis confirmed the presence of truncated protein in the testes of homozygous mutant males. (J:324057)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tesmin Mutation: 24 strains or lines available

References

• Original: J:324057 Zhang X, et al., Nuclear translocation of MTL5 from cytoplasm requires its direct interaction with LIN9 and is essential for male meiosis and fertility. PLoS Genet. 2021 Aug;17(8):e1009753
• All: 1 reference(s)