Polk^tm1.1Csk
Targeted Allele Detail

Summary

• Symbol: Polk^tm1.1Csk
• Name: polymerase (DNA directed), kappa; targeted mutation 1.1, Chugai Pharmaceutical Co Ltd
• MGI ID: MGI:7265039
• Synonyms: Polk^D197A-E198A, Polk^tm1.1Nmi
• Gene: Polk Location: Chr13:96617198-96679087 bp, - strand Genetic Position: Chr13, 50.56 cM, cytoband D1
• Alliance: Polk^tm1.1Csk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323839
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 6 was modified to introduce D197A and E198A mutations (GAC GAA to GCT GCA) and a floxed neo cassette was inserted in intron 5 via homologous recombination. Cre mediated recombination removed the neo cassette.The mutations in exon 6 result in expression in a catalytically-inactive protein. (J:323839)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polk Mutation: 65 strains or lines available

References

• Original: J:323839 Takeiri A, et al., In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C. DNA Repair (Amst). 2014 Dec;24:113-121
• All: 1 reference(s)