Tmprss2^{em2.1(TMPRSS2)Synbl}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmprss2^{em2.1(TMPRSS2)Synbl}
• Name: transmembrane protease, serine 2; endonuclease-mediated mutation 2.1, Synbal
• MGI ID: MGI:7261385
• Gene: Tmprss2 Location: Chr16:97365882-97412395 bp, - strand Genetic Position: Chr16, 57.53 cM, cytoband C2
• Alliance: Tmprss2^{em2.1(TMPRSS2)Synbl} page

Mutation origin

• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Tmprss2^{em2.1(TMPRSS2)Synbl} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TMPRSS2 (7113)

  Tmprss2

  (MGI:1354381)

• Mutation details: Cas9 and gRNA Tmprss2-crRNA_5 (protospacer sequence 5-GTGCTCAGACTGATACCCG-3) are used to introduce the following sequences at codon 19 in exon 3 of the mouse Tmprss2 gene: GGSG linker, P2A, human TMPRSS2 cDNA coding sequence with SV40, 1837 bp human TMPRSS2 3UTR, 112 bp human intergenic sequence immediately 3 of TMPRSS2 polyadenylation region, cassette comprised of a mouse U6 promoter-driven Tmprss2-gRNA5 guide RNA with 2xPolIII terminator, all in reverse orientation relative to Tmprss2 gene, splice acceptor and SV40 polyadenylation sequence designed to trap and truncate any mouse Tmprss2 isoforms that may splice past the hTMPRSS2 cassette. FRT-flanked PGK neomycin cassette with a bGHpA. The FRT sites allow Flp-mediated removal of the neomycin cassette from targeted embryonic stem cell clones as well as collapse of targeted tandem integration events if required. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tmprss2 Mutation: 46 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)