Ace2^{em1(ACE2)Synbl}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ace2^{em1(ACE2)Synbl}
• Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 1, Synbal
• MGI ID: MGI:7261373
• Gene: Ace2 Location: ChrX:162922338-162971414 bp, + strand Genetic Position: ChrX, 76.12 cM, cytoband F5
• Alliance: Ace2^{em1(ACE2)Synbl} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Ace2^{em1(ACE2)Synbl} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ACE2 (59272)

  Ace2	(MGI:1917258)

• Mutation details: Enhanced specificity eSpCas9 and gRNA Ace2-cRNA_32 (protospacer sequence 5'-AAGGCTGAGAAGGAGCCAGG-3') are used to introduce the following sequence 13 bp downstream of the mouse Ace2 start codon in exon 2: signal peptide coding sequence (translation produces amino acid sequence Ace2 WLLLSLVAVTTA) in-frame with first 5 amino acids of mouse Ace2, human ACE2 cDNA coding sequence with SV40 intron inserted for enhanced expression, 872 bp human ACE2 3' UTR, 100 bp human intergenic sequence immediately 3' of ACE2 polyadenylation region, cassette comprised of a mouse U6 promoter-driven Ace2-gRNA32 guide RNA with 2xPolIII terminator, all in reverse orientation relative to Ace2, splice acceptor and SV40 polyadenylation sequence followed by a Flp Recombinase Target (FRT) site and 3-frame ATG short open reading frames. This cassette is designed to trap and truncate any mouse Ace2 isoforms that may splice past the hACE2 cassette. The FRT site allows Flp-mediated collapse of targeted tandem integration events if required. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Ace2 Mutation: 54 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)