Ramp1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ramp1^{tm1c(EUCOMM)Wtsi}
• Name: receptor (calcitonin) activity modifying protein 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7260204
• Synonyms: RAMP1^fl
• Gene: Ramp1 Location: Chr1:91107544-91152918 bp, + strand Genetic Position: Chr1, 46.08 cM
• Alliance: Ramp1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ramp1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:322306
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91124046 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 91124855. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:322306)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ramp1 Mutation: 20 strains or lines available

References

• Original: J:322306 Ding W, et al., Regulation of Cutaneous Immunity In Vivo by Calcitonin Gene-Related Peptide Signaling through Endothelial Cells. J Immunol. 2022 Feb 1;208(3):633-641
• All: 6 reference(s)