Gt(ROSA)26Sor^{em2(CAG-Brainbow2.1,-Kras*)Koo}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-Brainbow2.1,-Kras*)Koo}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, Bon-Kyoung Koo
• MGI ID: MGI:7259836
• Synonyms: Red2-Kras^G12D
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-Brainbow2.1,-Kras*)Koo} page

Mutation origin

• Strain of Origin: B6.129P2-Gt(ROSA)26Sor^{tm1(CAG-Brainbow2.1)Cle}/J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted the Red2Onco construct, containing Kras^G12D (addgene 11549), into the tdimer2 reporter within the Gt(ROSA)26Sor^{tm1(CAG-Brainbow2.1)Cle}. The Brainbow2.1 (also known as Confetti) reporter construct is a modification of the Brainbow reporter with a strong CAGG promoter, a loxP site, a PGK-Neo-pA cassette (serving as a transcriptional roadblock), and coding region composed of two adjacent floxed head-to-tail tandem dimers. The first head-to-tail dimer contains a loxP site and humanized Renilla GFP (hrGFPII; with nuclear localization signal plus polyA sequence) in forward orientation and a loxP site and monomeric EYFP (mYFPA206K plus polyA sequence) in reverse orientation. The second head-to-tail dimer contains a loxP site and tdimer2 RFP plus polyA sequence in forward orientation, and a loxP site and mCerulean CFP (with membrane tethering palmitoylation sequence plus polyA sequence) in reverse orientation. A single frt site is located at the 3' end of the reporter construct. (J:322623)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:322623 Yum MK, et al., Tracing oncogene-driven remodelling of the intestinal stem cell niche. Nature. 2021;594(7863):442-447
• All: 4 reference(s)