Pnkp^em1.1Bie
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pnkp^em1.1Bie
• Name: polynucleotide kinase 3'- phosphatase; endonuclease-mediated mutation 1.1, Jeff Biernaskie
• MGI ID: MGI:7258498
• Synonyms: Pnkp^fl
• Gene: Pnkp Location: Chr7:44506563-44512416 bp, + strand Genetic Position: Chr7, 28.94 cM, cytoband B2
• Alliance: Pnkp^em1.1Bie page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:322582
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: Zinc-finger nucleases and a targeting donor RNA were used to insert loxP sites surrounding exon 2 and an FRT flanked neo cassette downstream of the second loxP site. Flp mediated recombination removed the neo cassette. (J:322582)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pnkp Mutation: 36 strains or lines available

References

• Original: J:322582 Shin W, et al., PNKP is required for maintaining the integrity of progenitor cell populations in adult mice. Life Sci Alliance. 2021 Sep;4(9)
• All: 1 reference(s)