Rnf31^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnf31^{tm1c(KOMP)Wtsi}
• Name: ring finger protein 31; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7257821
• Gene: Rnf31 Location: Chr14:55829199-55841131 bp, + strand Genetic Position: Chr14, 28.19 cM
• Alliance: Rnf31^{tm1c(KOMP)Wtsi} page
• IMPC: Rnf31 gene page

Mutation origin

• Mutant Cell Line: EPD0180_3_F09
• Germline Transmission: Earliest citation of germline transmission: J:322048
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55831290 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55834603. FLP excised removing the neo selection cassette and lacZ reporter leaving behind the floxed critical exon(s). (J:322048)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rnf31 Mutation: 48 strains or lines available

References

• Original: J:322048 The Centre for Phenogenomics, Direct Data Submission for The Centre for Phenogenomics Alleles. MGI Direct Data Submission. 2022
• All: 1 reference(s)