Hdgfl2tm1c(KOMP)Wtsi
Targeted Allele Detail
|
Symbol: |
Hdgfl2tm1c(KOMP)Wtsi |
Name: |
HDGF like 2; targeted mutation 1c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:7257818 |
Gene: |
Hdgfl2 Location: Chr17:56386634-56407607 bp, + strand Genetic Position: Chr17, 29.15 cM, cytoband D
|
Alliance: |
Hdgfl2tm1c(KOMP)Wtsi page
|
IMPC: |
Hdgfl2 gene page |
|
Mutant Cell Line: |
EPD0399_5_A03 |
Germline Transmission: |
Earliest citation of germline transmission:
J:322048
|
Parent Cell Line: |
JM8A3.N1 (ES Cell)
|
Strain of Origin: |
C57BL/6N-Atm1Brd
|
Project Collection: |
KOMP-CSD
|
|
Allele Type: |
|
Targeted (Conditional ready, No functional change) |
Mutation: |
|
Insertion
Vector: L1L2_Bact_P
|
|
|
Mutation details: The L1L2_Bact_P cassette was inserted at position 56403472 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 56405115. The critical exon(s) is/are thus flanked by loxP sites. FLP excised removing the neo selection cassette and lacZ reporter leaving behind the floxed critical exon(s).
(J:322048)
|
|
|
|
Original: |
J:322048 The Centre for Phenogenomics, Direct Data Submission for The Centre for Phenogenomics Alleles. MGI Direct Data Submission. 2022; |
All: |
1 reference(s) |
|