Mgl2^{tm1.1(cre)Aiwsk}
Targeted Allele Detail

Summary

• Symbol: Mgl2^{tm1.1(cre)Aiwsk}
• Name: macrophage galactose N-acetyl-galactosamine specific lectin 2; targeted mutation 1.1, Akiko Iwasaki
• MGI ID: MGI:7254996
• Synonyms: Mgl2-cre
• Gene: Mgl2 Location: Chr11:70021155-70028376 bp, + strand Genetic Position: Chr11, 42.99 cM
• Alliance: Mgl2^{tm1.1(cre)Aiwsk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:321812
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Mgl2^{tm1.1(cre)Aiwsk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mgl2 (MGI:2385729)

• Mutation details: The targeting vector contains an IRES-EGFP-cre-polyA cassette followed by an FRT-flanked neomycin resistant cassette (in reverse orientation) inserted into the 3' untranslated region (downstream of the stop codon in the last coding exon (exon 10). Flp-mediated recombination removed the FRT-flanked neo cassette. EGFP is not detectable by flow cytometry, and is not believed to be visible by direct fluorescence. Sporadic low-level expression of cre is observed in germ cells or during early embryogenesis. In addition, homozygous mice have little to no expression of MGL2 protein. (J:321812)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mgl2 (mouse) Summary of all recombinase alleles driven by Mgl2. Alliance mouse Mgl2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mgl2 Mutation: 27 strains or lines available

References

• Original: J:321812 Tatsumi N, et al., Effective CD4 T cell priming requires repertoire scanning by CD301b(+) migratory cDC2 cells upon lymph node entry. Sci Immunol. 2021 Dec 10;6(66):eabg0336
• All: 1 reference(s)