Gt(ROSA)26Sor^{tm1.1(CAG-iRFP*/mScarlet-I*/mTq2*/mVenus*)Rva}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-iRFP*/mScarlet-I*/mTq2*/mVenus*)Rva}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Renee van Amerongen
• MGI ID: MGI:7254850
• Synonyms: Rosa26^PRIME
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-iRFP*/mScarlet-I*/mTq2*/mVenus*)Rva} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contains (from 5' to 3') a strong CAGG (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid) promoter, three pairs of heterospecific lox sites (loxp, lox2272 and loxN), and the PRimary colours In the MEmbrane (PRIME) construct (described in greater detail below), followed by an FRT-flanked neomycin resistance (neo) cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation into B6_ColA_RMCE embryonic stem (ES) cells. Flp-mediated recombination removed the FRT-flanked neo cassette. The Rosa(PRIME) construct contains three pairs of heterospecific lox sites that are inserted separating four fluorescent proteins: a nuclear near-infrared fluorescent protein (3xNLS-iRFP670), membrane-bound red fluorescent protein (M-mSc-I), membrane-bound cyan fluorescent protein (M-mTq2), membrane-bound yellow fluorescent protein (M-mVen). iRFP670 is constitutively expressed in all cells. To ensure that only one fluorescent protein is expressed at any one time, an SV40 polyadenylation signal was inserted downstream of each open reading frame. Upon Cre activation, the lox sites recombine to create three mutually exclusive excision possibilities with the fluorescent protein that is placed first after the CAG promoter being expressed. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

Notes

6_ColA_RMCE embryonic stem (ES) cells

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)