H2ax^em1Shnk
Endonuclease-mediated Allele Detail

Summary

• Symbol: H2ax^em1Shnk
• Name: H2A.X variant histone; endonuclease-mediated mutation 1, Satoshi H Namekawa
• MGI ID: MGI:6890376
• Synonyms: H2ax-Y142A
• Gene: H2ax Location: Chr9:44246012-44247374 bp, + strand Genetic Position: Chr9, 24.84 cM
• Alliance: H2ax^em1Shnk page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to create a tyrosine to alanine substitution at position 142 (Y142A, TAC to GCT) and a G to A substitution two bases downstream (creating an AflII target site). The mutation disrupts phosphorylation of Y142. (J:321790)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2ax Mutation: 6 strains or lines available

References

• Original: J:321790 Abe H, et al., The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis. Curr Biol. 2020 Feb 3;30(3):408-420.e5
• All: 1 reference(s)