Agxt^em1Yech
Endonuclease-mediated Allele Detail

Summary

• Symbol: Agxt^em1Yech
• Name: alanine-glyoxylate aminotransferase; endonuclease-mediated mutation 1, Y Eugene Chen
• MGI ID: MGI:6888018
• Gene: Agxt Location: Chr1:93062962-93073143 bp, + strand Genetic Position: Chr1, 47.0 cM
• Alliance: Agxt^em1Yech page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 1-bp deletion in exon 1, an A three bases from the photospacer adjacent motif (PAM) resulting in a frame-shift mutation after amino acid 53 of the open reading frame and introducing a premature stop codon. Western blot analysis confirmed the absence of protein in liver of homozygotes. (J:304415)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agxt Mutation: 20 strains or lines available

References

• Original: J:304415 Rom O, et al., Glycine-based treatment ameliorates NAFLD by modulating fatty acid oxidation, glutathione synthesis, and the gut microbiome. Sci Transl Med. 2020 Dec 2;12(572)
• All: 2 reference(s)