C2cd3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: C2cd3^{tm1c(EUCOMM)Wtsi}
• Name: C2 calcium-dependent domain containing 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6887626
• Synonyms: C2cd3^ex4-5fl
• Gene: C2cd3 Location: Chr7:100021440-100119359 bp, + strand Genetic Position: Chr7, 54.35 cM
• Alliance: C2cd3^{tm1c(EUCOMM)Wtsi} page
• IMPC: C2cd3 gene page

Mutation origin

• Mutant Cell Line: EPD0682_5_B05
• Germline Transmission: Earliest citation of germline transmission: J:308674
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 100038825 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 100040673. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:308674)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C2cd3 Mutation: 101 strains or lines available

References

• Original: J:308674 Chang CF, et al., Centriolar Protein C2cd3 Is Required for Craniofacial Development. Front Cell Dev Biol. 2021;9:647391
• All: 2 reference(s)